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. Author manuscript; available in PMC: 2009 Nov 26.
Published in final edited form as: Brain Behav Immun. 2008 Jun 17;22(8):1138–1145. doi: 10.1016/j.bbi.2008.06.003

Figure 3. GH mRNA and protein expression in the pituitary gland of Ghsr-/- is reduced compared to wild-type control mice.

Figure 3

(A) GH mRNA levels are assessed using real-time RT-PCR using RNA isolated from the in pituitary glands of young (2m) and old (24m) female Ghsr-/- and Ghsr+/+ wild-type control mice. The data are expressed as fold change GH mRNA (+/- SD) compared to the young wild-type control mice. P values indicate significant differences. (n = 4-5 mice per group). In comparing GH mRNA expression between young and old Ghsr-/- mice, there is no significant difference between these mice with age (P=0.121). Similarly, there is no significant difference with age in the wild-type mice (P=0.251). However, there are significant differences in GH mRNA expression between the wild-type and GHSR-/- mice at the younger (P=0.0257) and older (P=0.0187) ages. [2-way ANOVA, Age: p = 0.207; Genotype p < 0.001; Tukey Kramer method: Genotype p < 0.001 Control vs Ghsr-/-] (B) Immunohistochemical analysis of GH protein expression in sections of pituitary glands of young (2m) and aged (24m) female Ghsr-/- compared to wild-type Ghsr+/+ mice. (n = 3 mice per group were examined)