Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 Aug 18;241(2):163–172. doi: 10.1016/j.taap.2009.08.010

Figure 7.

Figure 7

Pc4 and Pc4SNP induced apoptotic and necrotic death in A375 cells after irradiation. (a–e) Cells were seeded in individual wells of a 6-well plate, pretreated with Pc4SNP of different concentrations in DMEM (FBS free) for 2 hours, and then exposed to the light (15 J/cm2). After irradiation, the cells were incubated overnight in cell culture medium (2% FBS) and then stained with Annexin V-FITC and propidium iodide. Apoptotic and necrotic cell death were determined with flow cytometry. For NaN3 protection assay (f), cells were incubated with 20 mM NaN3 during the incubation period as well as the irradiation period. (g) The graphs illustrate photo-induced Pc4 and Pc4SNP dose-dependent apoptosis and necrosis and the protection effect by NaN3.