Fig. 5.
Specificity of 4.1 effector proteins to glutamate receptor recruitment. (A) Protein 4.1B did not significantly enhance recruitment of AMPA type receptors (GluR1) as compared to NR1/NR2B type receptors (arrowhead). Scale bar equals 2 μm. (B) Protein 4.1N was recruited to sites of HA-SynCAM1 accumulation in contact with microspheres (arrowheads). (C) Protein 4.1N induced significant recruitment of GluR1, but not NR1/NR2B, containing receptors to adhesion sites at microspheres (arrowheads). (D) Quantification of GluR1 recruitment vs. NR1/NR2B recruitment via protein 4.1B (34.9 ± 16.8% vs. 148.7 ± 13.3%, p < 0.001, n = 15). (E) Quantification of GluR1 recruitment vs. NR1/NR2B recruitment via protein 4.1N (139.8 ± 24.4% vs. 33.4 ± 6.95%, p < 0.001, n = 15). Error bars represent s.e.m.