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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Mol Cell Neurosci. 2009 Sep 29;42(4):466–483. doi: 10.1016/j.mcn.2009.09.010

Fig. 6.

Fig. 6

Localization of endogenous protein 4.1B in cultured hippocampal neurons. (A) Immunolabeling of protein 4.1B (green) with the synaptic markers PSD-95 (red) and synapsin I (blue) at 4, 8 and 12 DIV. Arrowheads indicate examples of protein 4.1B localization. Synaptic sites, areas enriched in all three proteins are white in the merged image (bottom row). Scale bar equals 10 μm. (B) Quantification of the percentage of synapses that show protein 4.1B localization over time in culture. Error bars represent s.e.m. (C) Quantification of the distribution of protein 4.1B puncta as determined by colocalization with synapsin I, PSD-95, both (Synaptic) or neither markers. Error bars represent s.e.m. (* p < 0.05, ** p < 0.01, n = 10).

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