Table 2.

Peak-to-peak correlation (as a percentage of total OTUs) between measured TRFLP patterns and predicted TRFLP patterns from an in silico digestion of 16S rRNA clone library data.

	South Rift		Pisces Peak
	15 f.u. cut-off	50 f.u. cut-off	15 f.u. cut-off	50 f.u. cut-off
TRFLP peaks perfect match	12 ± 4	8 ± 10	42 ± 4	56 ± 11
TRFLP peaks off by 1–2 bp	25 ± 13	37 ± 29	30 ± 4	32 ± 12
TRFLP peaks not matched	63 ± 12	55 ± 20	28 ± 3	12 ± 7
TRFLP ‘members’ identified	67 ± 11	75 ± 9	92 ± 3	93 ± 3
in silico peaks perfect match	24 ± 13	6 ± 8	29 ± 4	15 ± 4
in silico peaks off by 1–2 bp	45 ± 15	20 ± 15	21 ± 4	8 ± 3
in silico peaks not matched	30 ± 7	77 ± 3	50 ± 6	77 ± 3

Data are presented for the correlations when the measured TRFLP fragments are assumed versus both 15- and 50-fluorescence-unit (f.u.) threshold cut-off values.