Table 1. Summary of Dependent and Independent Variables.

Vari-able	Equivalent	Comments
B1		Mole fraction of Tm subunits coupled to the Tn-actin interaction and no calcium bound. Tm in Position B.
B2	;	Mole fraction of Tm subunits coupled to the Tn-actin interaction and one calcium bound. Tm in Position B.
B3	;	Mole fraction of Tm subunits coupled to the Tn-actin interaction and two calcium bound. Tm in Position B.
B−		Mole fraction of Tm subunits coupled to the mutant Tn-actin complex. Tm in Position B.
T1	;	Mole fraction of Tn dissociated from actin and tethered to Tm; no calcium bound. Tm position is undefined.
T2		Mole fraction of Tn dissociated from actin and tethered to Tm; one calcium bound. Tm position is undefined.
T3		Mole fraction of Tn dissociated from actin and tethered to Tm; two calcium bound. Tm position is undefined.
T−		Mole fraction of mutant Tn dissociated from actin and tethered to Tm.
C		Mole fraction of Tm directly associated with actin. Tm at equilibrium in Position C.
M		Mole fraction of Tm subunits coupled to the myosin-actin interaction. Tm in Position M.
PM	M	Probability of myosin coupled to the work associated with Tm in Position M; 0≤PM≤1
Ca		Calcium concentration; continuously independent variable.