Figure 2.

Western analysis of VEGF and VEGF receptor expression in HN33 cells. Protein from control cultures (Con) or from cultures exposed to HGD for 4 h was probed for candidate VEGF receptors with monoclonal (anti-VEGF and anti-VEGFR-2/Flk-1) or affinity-purified polyclonal (anti-VEGFR-1/Flt-1 and anti-neuropilin-1) antibodies, as described in Materials and Methods. Hippocampal tissue taken 72 h after 15 min of global cerebral ischemia (72 h/GI) was used as a positive control for VEGFR-1. The experiment was repeated three times with similar results.