Figure 3.

Effects of VEGF family members (PlGF-2 and VEGF) and of PI3-K inhibitors (wortmannin and LY294002) on HN33 cell viability after HGD. Cultures were exposed to control conditions (cell viability = 90 ± 2%, n = 3; not shown) or to HGD for 24 h, in the absence of added factors (HGD), or in the presence of 300 ng/ml of PlGF-2, 100 ng/ml of VEGF, 100 ng/ml of VEGF + 30 nM wortmannin (WM), or 100 ng/ml of VEGF + 10 μM LY294002 (LY). Cell viability was measured as described in the legend to Fig. 1. Data shown are mean values ± SEM from a representative experiment, performed in triplicate, which was repeated three times with similar results. PlGF-2 also failed to increase viability at 10, 50, or 100 ng/ml, and neither wortmannin nor LY294002, added alone, altered the viability of control or HGD-treated cells (not shown). The asterisk indicates P < 0.05 by ANOVA and Student–Newman–Keuls tests relative to all other conditions. The experiment was repeated three times with similar results.