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. Author manuscript; available in PMC: 2010 Nov 24.
Published in final edited form as: Biochemistry. 2009 Nov 24;48(46):10866–10881. doi: 10.1021/bi901476k

Figure 2.

Figure 2

Experiments contain 400 nM HSV-1 primase, 2 μM template, 0.8 mM [α-32P]GTP and 0–2 mM of investigated NTPs. Panel. The noted concentration of GTP and the template T20GC3[2-pyridone]AT14. Panel B. The noted concentration of CTP and the template T20GC3GAT14.Panel C. The noted concentration of UTP and the template T20GC3GAT14.Panel D. Incorporation of various NTPs opposite purine in the template T20GC3[purine]AT14. Reactions contained 1 mM NTP analogue. Lane 1) no analogue; 2) benzimidazole; 3) 4-methyl-2-pyridone; 4) 7-methylbenzimidazol; 5) 2-pyridone; 6) 6-methyl-1-deazapurine; 7) 1-deazapurine; 8) isoG; 9) purine; 10) 2(1H)-pyrimidinone. Presence of products of altered electrophoretic mobility indicates analog’s incorporation.