Figure 4.

Detecting primer/template slippage during misincorporation. Reactions were performed as described under Experimental Procedures and contained 400 nM HSV-1 primase and 2 μM template T₂₀GC₂TXAT₁₄ and X is 2-pyridone, unless noted otherwise. Primer standards of known composition were generated in assays containing T₂₀GCCCTAAT₁₄ and 0.6 mM [α-³²P]GTP (Lane S1). T₂₀GCCTTAAT₁₄ 0.6 mM [α-³²P]GTP and 0.6 mM ATP (Lane S2) or T₂₀GC₂T₂A₂T₁₄, and 0.6 mM [α-³²P]GTP (Lane S3). The identity of the products is listed on the sides of the panels. Panel A. Reactions contained 0.6 mM GTP and 0.2–1mM of [α-³²P]ATP. Panel B. Reactions contained 0.6 mM [α-³²P]GTP and 0–1mM of ATP. Panel C. Reactions contained 0.6 mM ATP and 0.2–1mM of [α-³²P]GTP.