Figure 1.

The neurological response of EAE mice treated with or without Tbeta4. The significant therapeutic Tbeta4 effects were detected as early as day 11 after EAE onset. Nearly 50% relative functional recovery was observed in the Tbeta4 treated group, compared to the saline controls with p<0.01 for either the median score or the cumulative score up to 30 days. B~D. H&E staining show inflammatory infiltrates adjacent to vessels (black arrows) in the brain of EAE control mice (B) and Tbeta4 treated mice (C). Quantitative data (D) show the numbers of vessels containing inflammatory infiltrates were significantly reduced in the Tbeta4 treatment group compared with the saline group (p<0.05). EµI. The DAB staining by NG2 shows the OPCs in the SVZ (E~F) and the white matter (G~H) of EAE mice treated by saline (E, G), and Tbeta4 (F, H). Quantitative data show that NG2⁺ cells (I) were significantly increased at 30d after EAE onset in the Tbeta4 treatment group compared to that in the saline group. (J~L) The staining by CNPase shows the mature oligodendrocytes in the white matter of EAE mice treated by saline (J) and Tbeta4 (K). Quantitative data (L) show that CNPase⁺ cells were significantly increased at 30d after EAE onset in the Tbeta4 treatment group compared to that in the saline group. (M~Q) Double immunofluorescence staining (nuclei, DAPI, blue) indicated that BrdU⁺ cells (FITC, green) were colocalized with NG2⁺ OPCs (CY3, red) in the corcpus collasum (M), SVZ (N) and striatum (O), BrdU⁺ cells were also colocalized with CNPase⁺ mature oligodendrocytes (CY3, red) in the white matter (P). Quantitative data (Q) show that BrdU⁺-NG2⁺ cells and BrdU⁺-CNPase⁺ were significantly increased at 30d after EAE onset in the Tbeta4 treatment group compared to that in the saline group. Scale bars in B~C=50 µm, E~H, J~K, M~P=25 µm.