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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Pharmacol. 2009 Aug 4;79(1):57–66. doi: 10.1016/j.bcp.2009.07.016

Fig. 4. Levels of hepatic protein ubiquitination in wild-type and Cyp2e1-null mice following APAP exposure.

Fig. 4

Equal amounts of liver cytosolic proteins (40 μg/well) from different groups were separated on 12% SDS–PAGE, transferred to nitrocellulose membranes, and subjected to immunoblot analysis by using anti-ubiquitin (A and B, upper panels) and anti-β-actin antibody (A and B, middle panels) for normalization. Coomassie-stained gels are also presented (A and B, lower panels) to further demonstrate equal protein loading. The relative ratio of the 3-NT level detected between the APAP-exposed samples and the corresponding control, which was set at 1, is shown in the top.