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. Author manuscript; available in PMC: 2009 Nov 26.
Published in final edited form as: Hepatology. 2009 Nov;50(5):1567–1576. doi: 10.1002/hep.23146

Figure 5.

Figure 5

Depletion of β-catenin decreases GJIC and expression of Cx43 in WB-F344 cells. (A) Cells were transfected with control or β-catenin-specific siRNA for 48 or 72 h, followed by determination of GJIC. Data are means ± SEM of three (48 h) or means of two (72 h) independent experiments. (B) Western analysis of β-catenin and connexin-43 levels in WB-F344 cells treated with β-catenin-specific siRNA for 48 or 72 h. Cx43 protein levels as determined by densitometric analyses were related to GAPDH levels. Cx43/GAPDH ratios are given as means ± SEM (n=3), with the 48 h control set to 100. (C) Physical interaction of β-catenin and Cx43 as demonstrated by co-immunprecipitation. Western blotting analysis of immunoprecipitates prepared from WB-F344 cell lysates (75 μg of total protein) employing anti-β-catenin or anti-Cx43 antibodies or a nonspecific rabbit IgG as negative control. Cell lysate (20 μg of protein) was analysed as input control.