Table 1.
Sequences of primer pairs and siRNAs employed in this study
| Target | Sequences 5′ → 3′ | Genbank entry |
|---|---|---|
|
PCR Primers | ||
| 18S rRNA | Gccgctagaggtgaaattcttg cattcttggcaaatgctttcg |
X01117 |
| β-actin | gtcgtaccactggcattgtg ctctcagctgtggtggtgaa |
NM_031144 |
| β-catenin #1 | ggccagtggtggaccccaagcct aggccccagtgcctgcatccca |
NM_053357 |
| β-catenin #2 | acgctgcataatctcctgct gagcttgctttcctgattgc |
NM_053357 |
| Cx43 #1 | ggcgtgaggaaagtaccaaa acagcgaaaggcagactgtt |
X06656, NM_012567 |
| Cx43 #2 | aacagtctgcctttcgctgt aaagcgagagacaccaagga |
X06656, NM_012567 |
| Cx43 #3 | agcctgaactctcatttttcctt ccatgtctgggcacctct |
X06656, NM_012567 |
| GAPDH | gatcgtggaagggctaatga ggatgcagggatgatgttct |
NM_017008 |
| HuR | ttcgggataaagttgcagga tttgcagtaacatagttcacaaagc |
XM_344063 |
| p21 | agcaaagtatgccgtcgtct ggcgcttggagtgatagaaa |
NM_080782 |
| SDHA | cgagatccgtgaaggaagag gcccatgttgtaatgcacag |
NM_130428 |
| siRNAs (supplier) | ||
| Ctrl #1 (Qiagen) | uucuccgaacgugucacguuu | |
| Ctrl #2 (Dharmacon) | ugguuuacaugucgacuaa | |
| HuR #1 (Qiagen) | aagaggcaauuaccaguuuca | XM_344063 |
| HuR #2 (Dharmacon) | caacaagucccacaaauaauu | XM_344063 |
| Cx43 (Smart Pool, Dharmacon) | caacaaccuggcugcgaaa, ugauugaaaugucgaguua, cgugaagggaagaagcgau, uuacugagauucugcgaua | NM_012567 |
| β-catenin (Smart Pool, Dharmacon) | gaucuuagcuuacggcaauuu, gggagaagcccuuggauuu, Aagcugaccucauggaguuuu, guagaaacagcccguuguauu | NM_053357 |