Fig. 6. Anticancer activity of Ads in DU145 tumor model.

(A) Subcutaneous DU145 tumors (average tumor volume 212 mm³) were injected once intratumorally with 100 µl of buffer or 3×10¹⁰ vp of Ad5, Ad6, Ad11, or Ad35 (n=6 or 7) in 100 µl volume on day 0. (top) Tumor volumes were measured twice a week. Average tumor volumes are shown up to the last day when all animals were alive in a group. Mice were euthanized when tumor volume reached 2000 mm³. Data presented as mean + standard error (SE). (bottom) Kaplan-Meyer survival curves for mice from (top) were plotted. (B) Mice bearing subcutaneously established DU145 tumors (average tumor volume 345 mm³) received two intravenous injections of 100 µl buffer or 3×10¹⁰ vp of Ad5, Ad6, Ad11, or Ad35 (n=7 or 8) in 100 µl volume on day 0 with 4 hours separating the injections. (top) Measurements of tumor volumes were taken twice a week. Average tumor volumes are shown up to the last day when all animals were alive in a group. Mice were euthanized when tumor volume reached 2000 mm³. Data presented as mean + SE. (bottom) Kaplan-Meyer survival curves for mice from (top) were plotted.