(A) CD46-transgenic C57BL/6 mice were injected intravenously with 100 µl of buffer or 1.5×1011 vp of Ad5, Ad6, Ad11, or Ad35 (n=3) in 100 µl volume on day 0. Blood was collected on day 3 after injection and serum ALT levels were measured. (B) Ads were preincubated with buffer or factor X for 10 min. at 37°C and then added to HepG2 cells at 1000 vp/cell. Two hours later the cells were washed and the medium was replaced. Cell viability was measured on day 3 after infection. Data presented as mean + SD. * - p=0.008, ** - p<0.001, n.s. – not significant.