Fig. 2.

Differences in population-level versus single-cell analysis of CLN2pr-GFP expression in yeast. (a) Production of GFP in ten WT yeast daughters throughout a time course. (b) Production of GFP in ten cln1Δcln2Δ yeast daughters throughout a time course. (c) Schematized plots of mean GFP fluorescence in all ten cells combined (top) and the fraction of individual cells out of ten in which GFP expression was induced (bottom), with numerical labels and vertical hash lines representing the three time points represented in (a) and (b).”Birth” is denoted as the disappearance of GFP-tagged myosin (represented by the green oval at the cell-to-cell junction) shown in the prior step. For simplicity, all ten daughters shown in (a) and (b) are not depicted as budding, although budding would normally be observed at steps 2 and 3. Plots in (c) are adapted from [19].