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. Author manuscript; available in PMC: 2010 Nov 27.
Published in final edited form as: J Mol Biol. 2009 Sep 23;394(2):219–225. doi: 10.1016/j.jmb.2009.09.043

Fig. 1.

Fig. 1

Position of Cys148 and paired-Cys replacements on the backbone of the X-ray crystal structure of LacY (PDB ID 2V8N). Left: side view; Cys148 (blue sphere; helix V); pairs are Ile40→Cys (green sphere; loop I/II) with Asn245→Cys (red sphere; helix VII); Pro31→Cys (green sphere; helix I) with Gln242→Cys (red sphere; helix VII) and Thr45→Cys (green sphere; helix II) with Gln242→Cys (red sphere; helix VII). Tandem fXa protease sites inserted between residue 136 and 137 are also shown in black. Right: viewed from the cytoplasm.