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. Author manuscript; available in PMC: 2010 Nov 27.
Published in final edited form as: J Mol Biol. 2009 Sep 23;394(2):219–225. doi: 10.1016/j.jmb.2009.09.043

Fig. 4.

Fig. 4

Effect of MTS cross-linking reagents on Cys148 labeling by MPB. Experiments were performed as described in Fig. 3 except that RSO vesicles containing mutant C148/I40C/N245C were treated with a given MTS cross-linking reagent at 0.1 mM (final concentration) for 30 min, and the reactions were stopped by adding 10 mM NEM. Treatment with MPB (475 μM) was carried out for given times at 0 °C.