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Ectopic expression of tbr::GFP BAC following mutation of a HesC site. (A) Map of tbr locus. The location of the HesC binding site (CGCGTG) in the α region is indicated. (B) Examples of ectopic expression in ectoderm, endoderm, and NSM of tbr::GFP BAC in which this site had been mutated, at 18, 24, and 48hpf. (C) Expression statistics for mutant and control BAC constructs as in figure 1D.
