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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: Dev Biol. 2009 Aug 11;335(2):428–441. doi: 10.1016/j.ydbio.2009.08.005

Fig. 4.

Fig. 4

Effects of deletion of C module from tbr::GFP BAC. (A) Map of tbrain locus. The C module was deleted by recombination (see Methods). (B) QPCR timecourse of GFP mRNA levels generated by the C module deletion, and by the parental tbr::GFP BAC. Sharply decreased expression is observed relative to the control tbr::GFP BAC. Error bars indicate SEM of repeated experiments. (C) Examples of GFP fluorescence image overlays showing embryos expressing the B module deletion, at 18, 24, and 48hpf. (D) Expression statistics for mutant and control BAC constructs as in figure 1D.