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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: Dev Biol. 2009 Aug 11;335(2):428–441. doi: 10.1016/j.ydbio.2009.08.005

Fig. 6.

Fig. 6

Expression of a minimal γ(2) module construct. A short construct consisting of the isolated γ(2) module associated with our standard endo16-GFP expression vector was constructed as described in Methods (construct γ(2)::EpGFP). (A) QPCR timecourse of GFP mRNA levels generated by γ(2)::EpGFP (red), and co-plotted with the calculated difference between the timecourse produced by the control tbr::GFP BAC and that produced by tbr::GFP BAC from which γ(2) module had been deleted (blue). All data are after normalization to the numbers of copies of the respective vectors incorporated at each timepoint, as above. . Error bars indicate SEM. (B) Examples of GFP fluorescence image overlays showing embryos expressing γ(2)::EpGFP, at 18, 24, and 48hpf. (C) Expression statistics for γ(2)::EpGFP together with control tbr::GFP BAC as in figure 1D.