Figure 3:

(a) Statistical analysis for genotypic differences of hearing phenotypes of Scn8a^Clth mice at 6, 10 and 18 weeks (n = 73), where F_t,u denotes the F-statistic on t degrees of freedom. Analysis was performed under a linear model (see Methods). Startle amplitudes and maximal latencies were measured in response to 40 ms soundbursts of white noise at 110 dB SPL using the startlebox paradigm. Parameters significantly different to wild types are given with the best fit of inheritance. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001 (unadjusted P values). (b) Statistical analysis for genotypic differences of ABR peak latencies of Scn8a^Clth mice at 5–7 months old, where F_t,u denotes the F-statistic on t degrees of freedom. Analysis was performed under a linear model (see Methods). Parameters significantly different to wild types are given with the best fit of inheritance. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001 (unadjusted P values). (c and d) Graphical representation of significantly different startle response and ABR peak latency parameters in Scn8a^Clth mice compared with wild types, showing data for adjusted P values < 0.05. Startle response was measured by placing mice in a soundproof chamber containing a loudspeaker to generate sound. Startle amplitude was calculated in arbitary units using an accelerometer connected to the chamber floor to measure floor displacement upon sound presentation. Data shown are estimate values for Scn8a^Clth/+ and Scn8a^Clth/Scn8a^Clth mice, having adjusted for sex and mating identifier, where +/+ is normalized to = 0.