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. 2009 Nov;9(7):1111–1122. doi: 10.1111/j.1567-1364.2009.00576.x

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© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Fig. 6 — qRT-PCR confirms microarray gene expression differences. qRT-PCR was used to analyze gene expression of immune-related genes in individual kidney samples. mRNA copy number for each gene was normalized to a GAPDH control. Data represent the mean of three individual samples (± SD) for each treatment group. White bars represent control kidneys; gray bars, attenuated strain-infected kidneys and black bars, virulent strain-infected kidneys. Means were compared by Kruskal–Wallis and Mann–Whitney-U-tests. For all genes, P<0.001 for control vs. virulent and attenuated vs. virulent. For control vs. attenuated, ^#P<0.05 for control vs. attenuated.