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. Author manuscript; available in PMC: 2010 Nov 3.
Published in final edited form as: FEBS Lett. 2009 Oct 12;583(21):3437–3442. doi: 10.1016/j.febslet.2009.10.013

Fig. 3.

Fig. 3

AA induced increase in MnSOD activity. A – Time-course of MnSOD activity in MECs after AA pretreatment (1.5µg/ml at indicated time points). Data are expressed as means±SE. B –Western blot analysis with anti-MnSOD antibody. C – In-gel zymography of MECs lysates for MnSOD activity before and after AAtreatment. D – Effect of cycloheximide (CH, 50µM) on MnSOD enzymatic activity. Note that AA (1.5µg/ml) caused time-dependent accumulation of catalytically active MnSOD tetramers (zymography, upper panel) compared to the amount of actin (Western blot, lower panel).