Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Oct 2;10(10):R104. doi: 10.1186/gb-2009-10-10-r104

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2009 Ling et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Strand-specific RT-PCR. Lanes 1 to 6 represent the amplification of the Sox4 and Sox11 transcripts using more than one probe. Lane 7 shows the amplification of the Hmbs housekeeping gene whereas lane 8 represents the amplification of water as non-template control (NTC) using the primer set for Hmbs. The first panel consists of amplicons generated from the reverse-transcribed sense strand cDNA whereas the second panel consists of amplicons generated from the reverse-transcribed antisense strand cDNA. The third panel represents amplicons generated from oligo-d [T]₁₅primed reverse-transcribed cDNA, which serves as a positive control. The last panel represents amplicons generated from primer-free reverse-transcription reactions. The numbers on the left indicate the size of the generated bands.