Table 4.
RT-qPCR validation of SAGE profile for P1.5 versus adult stages
| Fold change | ||||
|---|---|---|---|---|
| SAGE tag | RefSeq accession | Gene ID | Adult/P1.5 (SAGE) | Adult/P1.5 (RT-qPCR) |
| aaattattgggaaatcc | NM_011123 | proteolipid protein (myelin) 1, Plp1 | 38.9 | 43.33 |
| tttcagcagtgttggct | NM_013556 | hypoxanthine guanine phosphoribosyl transferase 1, Hprt1 | 6.99 | 2.26 |
| actcggagccaccagac | NM_009790 | calmodulin 1, Calm1 | 4.57 | 1.83 |
| gcttcgtccacacagcg | NM_010777 | myelin basic protein, Mbp | As | 1.79 |
| tccccgtcat | NM_026106 | down-regulator of transcription 1, Dr1 | Ps | -1.46 |
| gggaaactaagggagag | NM_172503 | zinc finger, SWIM domain containing 4, Zswim4 | -42.42 | -1.96* |
| gaacgcaagttcagccc | NM_031404 | actin-like 6B, Actl6b | -5.51 | -2.26 |
| gtgaaactaaaaaaaaa | NM_009094 | ribosomal protein S4, X-linked, Rps4x | -4.72 | -2.27 |
| Nil | NM_009726 | ATPase, Cu++ transporting, alpha polypeptide, Atp7a | Nil | -2.91 |
| agaagtgtttggagttt | NM_008253 | high mobility group box 3, Hmgb3 | -20.99 | -3.53 |
| cctttgtgacagtggcc | NM_025635 | ZW10 interactor, Zwint | 4.65 | -4.04† |
| acagtctatgttggagg | BQ177886/NM_010487 | C57BL/6 whole brain E15.5 (or known as embryonic lethal, abnormal vision, Drosophila-like 3 (Hu antigen C), Elavl3) | -41.99 | -4.06 |
| gatacttggaatgacta | NM_007393 | actin, beta, cytoplasmic, Actb | -18.49 | -4.35 |
| ctggcttctt | NM_008538 | myristoylated alanine rich protein kinase C substrate, Marcks | Ps | -4.76 |
| Nil | NM_025958 | cullin-associated and neddylation-dissociated 2 (putative), Cand2 | Nil | -5.62 |
| gctttgactgttctctt | NM_009870 | cyclin-dependent kinase 4, Cdk4 | -56.55 | -5.77 |
| ctcagtaatg | AA122503 | EST from M2 cells of skin melanoma | -12.28 | -11.12 |
| tggagcgttggctgtat | NM_009238 | SRY-box containing gene 4, Sox4 | -31.51 | -11.74 |
| cacgacaccccccaccc | NM_007792 | cysteine and glycine-rich protein 2, Csrp2 | Ps | -26.10 |
| tgtgtgaggtgttgtga | NM_009559 | zinc finger protein 57, Zfp57 | -16.33 | -32.74 |
| gggacctcgtggaagcc | NM_010025 | doublecortin, Dcx | -24.56 | -82.11 |
All RT-qPCR data are statistically significant at P < 0.001 unless specified: * P < 0.01. †A disagreement between RT-qPCR and SAGE data. As: adult-specific expression; Nil: SAGE data not available; Ps: P1.5-specific expression. Fold change values of <1.0 are presented in a negative fold change format.