Table 5.
RT-qPCR validation of SAGE profile for E15.5 versus P1.5 stages
Fold change | ||||
---|---|---|---|---|
SAGE tag | RefSeq accession | Gene ID | P1.5/E15.5 (SAGE) | P1.5/E15.5 (RT-qPCR) |
Atttctttggtgatttt | NM_010838 | microtubule-associated protein tau, Mapt | 5.51 | 1.53* |
Gcactgttaacaagtgt | NM_009234 | SRY-box containing gene 11, Sox11 | -2.17 | -3.32 |
All RT-qPCR data are statistically significant at P < 0.001 unless specified: * P < 0.05. Fold change values of <1.0 are presented in a negative fold change format.