Table 6.
RT-qPCR validation of SAGE profile for rostral E15.5 and caudal E15.5 regions
| Fold change | ||||
|---|---|---|---|---|
| SAGE tag | RefSeq accession | Gene ID | Caudal/rostral (SAGE) | Caudal/rostral (RT-qPCR) |
| gttgttcttccagtcgg | NM_016916 | bladder cancer associated protein homolog (human), Blcap | 2.68 | 1.43 |
| gtcatagctgttctgtg | BC025816 | EST sequence BC025816 | Cs | 1.31 (NS) |
| aagcttgacatttggaa | NM_026187 | ankyrin repeat and zinc finger domain containing 1, Ankzf1 | Rs | 1.17† (NS) |
| gatacttggaatgacta | NM_007393 | actin, beta, cytoplasmic, Actb | -2.63 | -1.32 |
| ttggtgaaggaaaaaac | NM_021278 | thymosin, beta 4, X chromosome, Tmsb4x | -2.33 | -2.13 (NS) |
All RT-qPCR data are statistically significant at P < 0.05 unless specified. †A disagreement between RT-qPCR and SAGE data. Cs: caudal region-specific expression; NS: no statistically significant difference between two developmental stages; Rs: rostral region-specific expression. Fold change values of <1.0 are presented in a negative fold change format.