Figure 4.

var2csa and Pf332 allele-specific transcriptional activity. (a, b) Transcriptional activity was confirmed for both var2csa allele types in HB3 and HB3CSA, and the single allele types of FCR3, FCR3CSA, NF54 and NF54CSA. (c) Both Pf332 copies in HB3 were also actively transcribed, demonstrating transcriptional functionality in these duplicated genes. Controls with RNA reverse transcribed without addition of reverse transcriptase (RT^-) and exchange of template for ddH₂O (NTC) were included in all experiments to prevent signals from gDNA or contaminations from influencing the interpretation of the results.