Table 1.
Primer and probe sequences
| Target gene (application) | Primers | Probes |
|---|---|---|
| seryl-tRNA synthesase (real-time PCR) | F: TATCATCTCAACAGGTATCTACATCTCCTA | NA |
| R: TTTGAGAGTTACATGTGGTATCATCTTTT | ||
| β-tubulin (real-time PCR) | F: CGTGCTGGCCCCTTTG | NA |
| R: TCCTGCACCTGTTTGACCAA | ||
| var2csa DBL2x (real-time PCR) | F: AATCATGGTGGAACACGAACAA | 6-FAM-ATATTTGGTTAGCAATGAA-MGB (allele 1) |
| R: AACTACTACCACTACCAGTGACACTACCAT | 6-VIC-ATATATTTGGACAGCAATGA-MGB (allele 2) | |
| var2csa DBL4ε (real-time PCR) | F: TATACCGTAAAAGTAACAAAGAATCGGAA | 6-FAM-ATGATTATGGAACCTACAGTC-MGB (allele 1) |
| R: ACAACTACAACAAATGTAGTTCCCATTAATT | 6-VIC-ATGATTATGGCACCTACAGT-MGB (allele 2) | |
| Pf332 S326P (real-time PCR) | F: AGTGGTTCCCAAGAAGAAGAACAA | 6-FAM-ATGATGTGTCACATAGGA-MGB (wild type) |
| R: ACATTCTGTCATCTAATTTATCCAGTAGAGAT | 6-VIC-ATGTGCCACATAGGA-MGB (Mutant) | |
| var2csa nest 1 (single cell PCR) | F: ACTTGAAAATGTGTGCAAAGGAGTA | NA |
| R: TTACCCAGTGGAGACGGAACAT | ||
| var2csa lr1 (DNA-FISH/Southern blot) | F: AAAATTACTGTGAATCATTCAGAT | NA |
| R: AAGGTGTTTCAGACGAAGTATTAGCAT | ||
| var2csa lr2 (DNA-FISH) | F: ACTGTGAATGTTACGAATTGTGGATAA | NA |
| R: ACATTTCCCCCTCAATTCCTTT | ||
| var2csa PFHG_05046.1_Sp6_a (RNA-FISH, detecting mRNA) | F: TCATTTAGGTGACACTATAGAAGTGTATATTTT GGGTCATCATTTCGATATTT |
NA |
| R: GAGATGAAAACAAGTGTGAAACAAAATC | ||
| var2csa PFHG_05046.1_Sp6_s (RNA-FISH, detecting antisense) | F: TCATTTAGGTGACACTATAGAAGAGATGAAAA CAAGTGTGAAACAAAATCC |
NA |
| R: TGTATATTTTGGGTCATCATTTCGAT | ||
| var2csa PFHG_05155.1_Sp6_a (RNA-FISH, detecting mRNA) | F: TCATTTAGGTGACACTATAGAAGTGCTTATAA TTTTCATCATCATTTGGATATTTATC |
NA |
| R: CAGATCAATTGAAAGGTTCTAACATTTT | ||
| var2csa PFHG_05155.1_Sp6_s (RNA-FISH, detecting antisense) | F: TCATTTAGGTGACACTATAGAAGAGATCAATT GAAAGGTTCTAACATTTTAGAAC |
NA |
| R: CTTATAATTTTCATCATCATTTGGATATTTATC | ||
| kahrp_Sp6_a (RNA-FISH, detecting mRNA) | F: TCATTTAGGTGACACTATAGAAGAGGTTGGTG AACCTGTGGTGCTT |
NA |
| R: AACTTTAGCACAAAAGCAACATGAA | ||
| kahrp_Sp6_s (RNA-FISH, detecting antisense) | F: TCATTTAGGTGACACTATAGAAGAGAACTTTA GCACAAAAGCAACATGAA |
NA |
| R: GTTGGTGAACCTGTGGTGCTT |
F, forward; NA, not applicable; R, reverse.