Figure 2.

Exposure of SCp2 cells to R. constantinopolitanus fraction I.2 at noncytotoxic concentrations reduces, in a concentration-dependent manner, ET-induced IL-6 levels. SCp2 cells were treated on day 3 of culture with EtOH (Ctrl) or I.2 at different concentrations in DM supplemented with 1% FBS: (A) Twenty-four hr later, trypan blue assay was performed. (B) Thirty min later, cells were treated with ET at 10 μg/ml and their media collected 9 hr post-ET. The values depicted are the means (± S.D.) of a triplicate treatment. Means with the same letter are not significantly different. Statistical significance of the difference from Ctrl+ET is with *P < 0.05, **P < 0.01 or ***P < 0.001. (Ctrl, control; ET, endotoxin; IL-6, interleukin-6). -ET (white square); +ET (black square).