Table 1.

Biochemical Binding Parameters from Solid Phase Assays

actin	Bmax (μM)	Kapp (μM)	R
α-G-	2.10 ± 0.07	0.031 ± 0.007	0.99
α-F-	2.31 ± 0.05	0.030 ± 0.004	0.99
β/γ-G-	1.90 ± 0.06	0.029 ± 0.005	0.99
β/γ-F-	2.01 ± 0.04	0.027 ± 0.003	0.99

Conditions were the same as those referred to in Figure 1. Binding parameters were estimated with the non-linear curve fit Hyperbl function (ΔA₄₅₀ = Bmax * [tau]/(Kapp+ [tau]) in the Origin program.