Table 2.

Description and results obtained for the Uruguayan isolates used for CGH and Caco-2 invasion assays

ISOLATE DESIGNATION	PHAGE TYPE	PERIOD OF ISOLATION	ORIGIN	RAPD-PCRa	PFGEb	CGHc P	CGHc A	SVPd	Caco-2 invasivenesse
31/88	UNTY	Pre epidemic	Coproculture	1	92%	32	43[4]	+	-
08/89	4b		Haemoculture	1	96%	33	41	+	-
53/94	4b		Food	0	100%	1	38	+	+
57/94	4		Food	0	100%	1	0[14]	+	+
47/95*	4	Epidemic	Coproculture	0	92%	2	0	+	+
51/95	4		Coproculture	1	100%	0	0	+	+
108/95*	4		Coproculture	0	100%	0	0	+	+
49/98	4		Food	0	100%	3	6[15]	+	+
80/98	4b		Bone punction	0	100%	0	1	+	+
100/99*	4		Coproculture	0	96%	0	0	+	+
130/99	4b		Coproculture	0	96%	0	0	-	-
132/99	4		Haemoculture	0	100%	0	0[33]	+	-
206/99	4b		Food	1	91%	0	45	-	+
32/00	4		Animal	0	100%	0	8[5]	+	+
125/00*	4		Coproculture	0	92%	0	0	+	+
48/01	4		Food	2	100%	0	0	+	-
251/01	RDNC		Egg	0	100%	0	0	+	-
254/01	4		Egg	0	96%	0	0	+	-
8/02*	4		Coproculture	0	100%	0	0	+	+
32/02	4		Egg	1	96%	0	1	-	+
65/02	4		Coproculture	0	100%	0	0	+	+
77/02*	4		Coproculture	0	100%	2	0	+	+
199/02	4		Haemoculture	1	ND	0	0	-	-
214/02	4		Haemoculture	1	96%	0	0	-	-
47/03	4		Coproculture	1	ND	0	6	+	+
106/04	4	Post epidemic	Coproculture	1	100%	0	0	+	+
10/05	RDNC		Coproculture	0	100%	2	0	+	+
92/05	4		Coproculture	0	96%	0	1[32]	+	+
93/05	4		Coproculture	0	100%	0	0	+	+

^a- The number of primers which showed differences in RAPD -PCR profiles as compared to S. Enteritidis PT4 P125109 is indicated.

^b- Expressed as percentage of identity as compared to S. Enteritidis PT4 P125109: 96% of identity corresponds to 1 band of difference, 92% to 2 bands and 91% to 3 bands of difference.

^c- P: number of chromosomal genes predicted as present in test isolate but absent in reference S. Enteritidis PT4 P125109. A: number of chromosomal genes predicted as absent in test isolate but present in S. Enteritidis PT4 P125109. Numbers between brackets indicate genes from the S. Enteritidis PT4 P125109 virulence plasmid lost or divergent in the tested isolate (total plasmid genes: 57).

^d- Results from Kado and Liu analysis [53]. +: Salmonella virulence plasmid present, -: absent.

^e- Results from Caco-2 invasion assays. -: invasiveness ≤ 30% of PT4, +: > 30% of PT4. All isolates marked with - showed significant differences compared to PT4 (p < 0,01). In Isolate Designation column, an * indicates strains previously reported [12].