Table 1.
Summary of novel human intronic 3'-end exon sites
| Site numbers | Genes | % all 3'-end exon sites | |
|---|---|---|---|
| i. Single-block ESTs | |||
| No. of poly(A/T)-tailed ESTs | 494,529 | ||
| No. of non-poly(A/T)-tailed ESTs | 2,829,147 | ||
| No. of final 3'-end exon sites | 10,844 | 5,670 | 100% |
| No. supported by non-poly(A/T)-tailed ESTs | 7,676 | 4,599 | 71% |
| ii. Validation tests | |||
| Blast hit against all known cDNAs | 2,957 | 2,257 | 27% |
| Support from EST clone IDs | 1,155 | 1,001 | 11% |
| Support from Affymetrix transcripts | 5,475 | 3,627 | 50% |
| RT-PCR experiments | 10/26 | ||
| iii. Overlapping with other AS and APA databases | |||
| PolyA_DB2 | 1,410 | 1,235 | 13% |
| ASAP II database | 613 | 554 | 6% |
| ASTD database | 1,250 | 1,115 | 11% |
| Muro et al's data | 4,046 | 2,895 | 37% |
| iv. PAS analysis | |||
| PAS positive | 7,051 | 4,292 | 65% |
| v. Map domains | |||
| At least one domain deletion | 7,641 | 4,142 | 70% |
| Transmembrane helix deletion | 1,616 | 945 | 15% |
See Results for details.