Table 5.
Primers used in the analysis of cell-wall-degrading enzymes
| Primers | Sequence (5' to 3') | Use |
|---|---|---|
| Eng1-0F | CTCTACGGGATGAAGTGTCT | In situ hybridization, amplification of cDNA and gDNA of Aa-eng-1 and Aa-eng-2 |
| Eng1-0R | TTAACAAAAGCGGTACAAG | In situ hybridization, amplification of cDNA and gDNA of Aa-eng-1 and Aa-eng-2 |
| Eng1-1F | GCTCAAGGTCGTCGTCGAGG | Sequencing of cDNA and gDNA of Aa-eng-1 and Aa-eng-2 |
| Eng1-10F | GGCATCTCCGAGGCCGACG | Sequencing of gDNA of Aa-eng-1 and Aa-eng-2 |
| Eng1-10R | CTTGCCGTACTCCTGCGCGAT | Sequencing of gDNA of Aa-eng-1 and Aa-eng-2 |
| Eng2-20R | GCTACTTTGCTGGTCCACGT | Sequencing of gDNA of Aa-eng-2 |
| Pel1-0F | TCCGACGACAACGTCAACCA | In situ hybridization, amplification of cDNA and gDNA of Aa-Pel-1 |
| Pe11-0R | AAACCCTCAGCATGTTTGATAC | In situ hybridization, amplification of cDNA and gDNA of Aa-Pel-1 |
| Pel1-1F | TCGAGAACGTCTGGTGGGA | Sequencing of cDNA and gDNA of Aa-Pel-1 and Aa-Pel-2 |
| Pe11-10F | CTTGGAGGTACGCTTCGTACG | Sequencing of gDNA of Aa-Pel-1 |
| Pe11-10R | TGACCTTCTTCGCCGCAGTG | Sequencing of gDNA of Aa-Pel-1 |
| Pe11-20F | GAAATGGTACGATTAGTCCTG | Sequencing of gDNA of Aa-Pel-1 |
| Pe12-0F | TCAGTCGGACAGCTTTTCCTC | Amplification of cDNA and gDNA of Aa-Pel-2 |
| Pe12-0R | AGCAGGCATTTCGTCGACAC | Amplification of cDNA and gDNA of Aa-Pel-2 |
| Pe12-10F | CGAGATGGCACGGGTGCCGA | Sequencing of gDNA of Aa-Pel-2 |
| Pe12-10R | CGTAGCGAGAAATTTTCGATCA | Sequencing of gDNA of Aa-Pel-2 |