Table 1.

List of parameters

Parameters	Value	Physical meaning	Source/reason
N	100	Number of legs.	Out of a total of 400 legs, one-quarter face the substrate.
Q	10	Number of binding sites on each foot.	Structural information: number of charges on the foot.
fm	0.39 pN	Motor force.	Fitting Eqs.1 and 2 with T-V data.
λ	28 nm	Power stroke length.	Structure of leg protein: 90° conformational change between two arms of 20 nm.
κ	80 pN/μm	Elastic constant of intermediate segment.	Physiological range.
kon, k′on	2.9 × 103 s−1	Binding rate of single site.	Fitting Eqs.1 and 2 with T-V data, assuming that the on-rates are not affected by external force.
koff	4.2 × 103 s−1(10.6 kBT)∗	Peel-off rate of single site (and its Arrhenius factor).	Fitting Eqs. 1 and 2 with T-V data.
k′off	1.9 × 103 s−1(16.8 kBT)∗	Weakly facilitated release rate of single site (and its Arrhenius factor).	Fitting Eqs. 1 and 2 with T-V data.

^∗Rates are listed as their values at the reference temperature 22.5°C.