Figure 2.

Expression of the γ subunit in Sf-9 cells and association with the α1 subunit. (A) An immunoblot of protein from cells infected with baculoviruses containing the cDNA for the human γ subunit or uninfected cells. The blots were probed with a γ specific polyclonal (γ969). (B) An immunoprecipitation of protein from metabolically labeled Sf-9 cells infected with α1, α1γ, or γ baculoviruses. Protein from α1-infected cells was immunoprecipitated with an α1-specific monoclonal (C464–6B). Protein from γ- and α1γ-infected cells was immunoprecipitated with γ969.