. Author manuscript; available in PMC: 2009 Dec 1.

Published in final edited form as: Anal Chem. 2008 Dec 1;80(23):9034–9041. doi: 10.1021/ac801417d

Table 1.

Myoglobin fragment number and sequence coverage for pepsin, protease type XIII, and protease type XVIII digestion, and subsequent separation with a reversed-phase HPLC C₅ column (see Figure 1). Protease type XIII yielded the most fragments with short average length compared to pepsin. Protease type XVIII produced the shortest fragments, but with low cleavage efficiency.

	Pepsin	Protease type XIII	Protease type XVIII
Number of Fragments	48	57	26
Sequence Coverage	96.7%	94.8%	80.4%
Average Length	15 aa	10 aa	9 aa
Optimal Protease Concentration	1.4 mg/mL	1.2 mg/mL	2.0 mg/mL