. Author manuscript; available in PMC: 2009 Dec 1.

Published in final edited form as: Anal Chem. 2008 Dec 1;80(23):9034–9041. doi: 10.1021/ac801417d

Table 2.

Myoglobin fragment assignment for pepsin and protease type XIII digestions, and subsequent separation with a Pro ZAP™ C₁₈ column (see Figure 2). Protease type XIII yields greater sequence coverage with shorter average peptide length than pepsin. Protease type XIII improves the sequence coverage and increases the accuracy of deuterium uptake assignment.

	Pepsin	Protease type XIII
Number of Fragments	77	96
Sequence Coverage	98.7%	99.8%
Average Length	17 aa	15 aa
Optimal Protease Concentration	1.4 mg/mL	1.2 mg/mL