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. Author manuscript; available in PMC: 2009 Nov 27.
Published in final edited form as: Cytometry A. 2008 Nov;73(11):1043–1049. doi: 10.1002/cyto.a.20594

Figure 4.

Figure 4

Comparison of CD137-enrichment to IFNγ secretion assay for enrichment of antigen-specific T-cells from a low precursor frequency. T-cells lines were induced by stimulating T-cells enriched for naïve T-cells by depletion of RO+ memory cells, with autologous dendritic cells, pulsed with the HCV NS3(1406–1415) peptide. After one week of culture in medium containing IL7 and IL15, each well was split and cells were processed either using the IFNγ secretion assay (Miltenyi) or the CD137-enrichment procedure. Analysis of each well by MHC-multimer-staining (Proimmune) was performed 7 days post enrichment. The irradiated negative flow-through was used as feeder cells. Each data point represents an enrichment procedure from an individual well. Results are pooled from 2 independent experiments.