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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Nat Immunol. 2009 Aug 2;10(9):1008–1017. doi: 10.1038/ni.1753

Figure 6. Nef inhibits class switching in GCs but not extrafollicular areas.

Figure 6

(a, b) Immunohistology of HIV-1− and HIV-1+ follicles from systemic lymph nodes stained for IgD (green), Nef (red), and CD11c (blue). DAPI (blue) counterstains nuclei. Arrowheads point to intercellular conduits. Mφ, macrophages. Original magnification, ×63. (c-e) HIV-1− and HIV-1+ follicles from systemic lymph nodes or intestinal Peyer's patches stained for IgD or IgA (green), AID, ferritin or BAFF (red), and Nef or CD68 (blue). DAPI (blue) counterstains nuclei. Original magnification, ×10. (f) ELISA of ferritin from uninfected or ADA-infected primary macrophages. (g) ELISAs of IgM, IgG and IgA from IgD+ B cells co-cultured with CD40L or BAFF and IL-10 in the presence or absence of ferritin for 7 d. Panels a-e show 1 of 4 experiments yielding similar results, whereas panels f and g summarize 3 experiments. Scale bar equals 10 μm.