Fig. 7.

Depression of intracellular calcium release prevents WIN from blocking NMDA-induced calcium influx in both DRG neurons and F-11 cells. A, pretreatment with SERCA pump inhibitor TG prevented WIN from blocking NMDA-induced calcium rise in both DRG neurons and F-11 cells. B, pretreatment with IP₃ receptor inhibitor 2-APB prevented WIN from blocking NMDA-induced Ca²⁺ signals in both cell types. Note, neither thapsigargin nor 2-APB alone produced any effect on NMDA-induced Ca²⁺ increase. *, p < 0.05 compared to NMDA treatment alone. †, p < 0.05 compared to NMDA + WIN treatment condition. The dotted line indicates the baseline calcium level.