Fig. 5.
Expression of GluA1–4 in hippocampus versus thalamus. P2 brain membrane fractions were separated in 4 to 12% Bis-Tris Criterion XT Precast gels (Bio-Rad) and transferred to polyvinylidene difluoride membranes. The blots were incubated with AMPA-R subunit-specific antibodies followed by treatment with IRDye-conjugated secondary antibodies (LI-COR). Blots were visualized and quantified by using the Odyssey imaging system (LI-COR). Two separate membrane preparations were analyzed. The AMPA-R signal was normalized to actin, and, for each subunit, all samples were quantified on the same blot. Statistical significance was determined by t test. The normalized values for individual subunits from hippocampus were compared to that of the corresponding receptor from thalamus (A1, p = 0.015; A2, p = 0.038; A3, p = 0.004; A4, p = 0.049). A, representative Western blot of AMPA-R subunits in hippocampus and thalamus of adult rats. B, average relative expression of AMPA-R subunits in thalamus compared to hippocampus. Error bars indicate the S.E.M.