TABLE 1.

E_max and EC₅₀ values for GluA/TARP combinations

Summary of the binding data from recombinant receptors expressed in HEK293 cells (Fig. 3). Binding to membranes from these cells was analyzed as described under Materials and Methods. Statistical significance was calculated by one-way ANOVA with least significant difference post hoc analysis. The E_maxvalues of each recombinant receptor were compared to those of the corresponding cotransfections with TARPs. To account for variance between assays (GluA2 and -4 sets), data were normalized to the mean of the control (A2 or A4 without TARP). For individual tests, the control E_max value was subtracted from the mean control E_max value. The difference was then applied to the E_max values of the cotransfections that were assayed in parallel with the control. ANOVA of these corrected values indicates that the differences are statistically highly significant [A2i/TARPs, F(4,20) = 6.6, P = 0.001; A4i/TARPs, F(4,15) = 4.5, P = 0.013]; the results of the post hoc analysis are shown in the table. Without normalization, some of the comparisons had P values above 0.05: A2/γ2 vs. A2/γ3 (P = 0.066), A4 vs. A4/γ3 (P = 0.17), and A4 vs. A4/γ8 (P = 0.11). For GluA2 alone and GluA4 alone, the S.E. shown in parenthesis was calculated before normalization.

GluA	EC50	Emax	N	Significance for Emax
				vs. GluA Alone	vs. Other TARPs
	μM	%		p =	p =
A1	2455 ± 1684	15 ± 4	3
A1/γ2	838 ± 237	16 ± 3	3	ns
A1/γ3	781 ± 208	23 ± 1	2	ns
A1/γ4	164 ± 11	16 ± 4	3	ns
A1/γ8	332 ± 124	14 ± 3	3	ns
A2	652 ± 105	99 (±7)	6
A2/γ2	563 ± 109	103 ± 8	4	ns
A2/γ3	603 ± 43	128 ± 7	6	0.001	0.011 vs. γ2
A2/γ4	719 ± 135	112 ± 7	5	ns
A2/γ8	724 ± 111	136 ± 8	4	<0.001	0.003 vs. γ2; 0.017 vs. γ4
A4	501 ± 106	101 (±13)	4
A4/γ2	508 ± 72	71 ± 8	4	0.002
A4/γ3	582 ± 108	84 ± 4	4	0.04
A4/γ4	655 ± 177	74 ± 4	4	0.003
A4/γ8	430 ± 98	81 ± 10	4	0.02

ns, not significant.