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Optimization of Epac-1 knockdown with anti-Epac-1 siRNA in murine macrophages. A, measurement of Epac-1 mRNA by RT-PCR normalized to 18S 48 h after electroporation with increasing concentrations of anti-Epac-1 siRNA. n = 3. B, measurement of Epac-1 protein expression by Western immunoblotting at the times indicated after electroporation with 400 nM anti-Epac-1 siRNA. n = 3.
