Scheme of the GlyR topology and the sequences of recombinant fragments. A, schematic topology of the human α1 GlyR subunit. B, alignment of intracellular fragments used for GST pull-down assays and molecular docking. Underlined are the sequences used for molecular modeling and docking. IL-WT, full α1 IL; NTIL-WT, N-terminal (17 amino acids) region of IL; NTIL-5A, mutant version with alanine substitutions; CFIL, central fragment (326–381) of IL; CTIL-WT, C-terminal region of IL; CTIL-2A, mutant version. The sequence of NTIL-WT served as template for the RQH peptide.