Fig. 4.
KU135-induced apoptosis occurs independently of the receptor-mediated apoptotic pathway. A, cells (5 × 105/ml) were cultured in the presence or absence of DMSO, 1 μM KU135, or 10 μM 17-AAG, harvested, and processed for cell death determination by flow cytometric analysis of annexin V-FITC and propidium iodide staining. Quadrants are defined as live (bottom left), early apoptotic (bottom right), late apoptotic (top right), and necrotic (top left). Numbers refer to the percentage of cells in each quadrant. B, duplicate aliquots of cells in A were harvested and lysed for Western blotting. Casp, caspase.