Fig. 1.
MGL inhibition prolongs DSE duration. A, time course of DSE in cells before (black triangles) and after (gray circles) treatment with a MGL inhibitor NAM (10 μM, 10 min). Right, bar graph illustrates t1/2 recovery half-life time courses for A. B, time course of DSE in cells before (black triangles) and after (gray circles) treatment with a selective MGL inhibitor JZL184 (100 nM, 10 min). Right, bar graph illustrates t1/2 recovery half-life time courses for B. C, time course in response to puffed 2AG (5 μM, 5 s) before (black triangles) and after (gray circles) treatment with JZL184 (100 nM, 10 min). Right, bar graph illustrates t1/2 recovery half-life time courses for C. D, bar graphs show relative EPSC charge after puffed CB1 agonist WIN (50 nM, 5 s) before or after treatment with JZL184 (100 nM, 10 min). E, sample EPSC time course shows that putative MGL inhibitor URB602 (20 μM) directly and reversibly inhibits EPSCs. F, the ABHD6 inhibitor WWL70 (10 μM, 10 min) has no effect on recovery time course of DSE. Right, bar graph illustrates t1/2 recovery half-life time courses for F. Error bars are 95% CI except D, which shows S.E.M..