TABLE 1.

Quantitation of FRET measurements by FRET^C/D and FRET^C/$\sqrt{\text{D}\times \text{A}}$ method

Values are presented as mean ± S.E.M.

Donor Proteins	Acceptor Proteins	FRETC/$\sqrt{\text{D}\times \text{A}}$ Average	FRETC/D Average
CFP-Myr	YFP-AC5	0.38 ± 0.09	0.38 ± 0.05
Cer-Gβγ	YFP-AC5	2.13 ± 0.20	1.61 ± 0.26
Cer-Gβγ	YFP-AC5Δ66–137	0.88 ± 0.24	0.64 ± 0.13